Overview

Description

  • Nature
    Recombinant
  • Source
    CHO cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      Signal peptide and extracellular domain of human DLL1 (aa 1-545) are fused at the C-terminus to the Fc portion of human IgG1.
    • Molecular weight
      100 kDa
    • Amino acids
      1 to 545

Specifications

Our Abpromise guarantee covers the use of ab108952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    ab108952 is 0.2µm filtered.
  • Form
    Lyophilised
  • Additional notes

    Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L-1 cells.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.2

  • Reconstitution
    Reconstitute in distilled water

General Info

  • Alternative names
    • Delta
    • Delta (Drosophila) like 1
    • Delta like 1
    • Delta like 1 (Drosophila)
    • Delta like 1 protein
    • Delta like protein 1
    • Delta like protein 1 precursor
    • Delta, Drosophila, homolog of 1
    • Delta-like protein 1
    • Delta1
    • DL1
    • DLL 1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    • OTTHUMP00000017690
    see all
  • Function
    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificity
    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similarities
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • Induction of Hes1 with the treatment of ab108952.
    A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5ug/ml of ab108952 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.
  • Interaction of Human Notch1 with Human DLL1. HEK293 cells transfected with a human Notch1 or a Human GITR ligand expressing vector were incubated with 25 ug/ml of Human GITR-Fc or ab108952). Cells were stained with anti-Human IgG(Fc specific) FITC conjugate for DLL1-Fc binding.

References

ab108952 has not yet been referenced specifically in any publications.

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