Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionO00548
    • SpeciesHuman
    • Molecular weight100 kDa
    • Amino acids1 to 545

Specifications

Our Abpromise guarantee covers the use of ab108952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityInhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L-1 cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 90 % SDS-PAGE.
    ab108952 is 0.2µm filtered.
  • FormLyophilised
  • Additional notes

    Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L-1 cells.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.2

  • ReconstitutionReconstitute in distilled water

General Info

  • Alternative names
    • Delta
    • Delta (Drosophila) like 1
    • Delta like 1
    • Delta like 1 (Drosophila)
    • Delta like 1 protein
    • Delta like protein 1
    • Delta like protein 1 precursor
    • Delta, Drosophila, homolog of 1
    • Delta-like protein 1
    • Delta1
    • DL1
    • DLL 1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    • OTTHUMP00000017690
    see all
  • FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant Human DLL1 protein images

  • Induction of Hes1 with the treatment of ab108952.
    A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5ug/ml of ab108952 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.
  • Interaction of Human Notch1 with Human DLL1. HEK293 cells transfected with a human Notch1 or a Human GITR ligand expressing vector were incubated with 25 ug/ml of Human GITR-Fc or ab108952). Cells were stained with anti-Human IgG(Fc specific) FITC conjugate for DLL1-Fc binding.

References for Recombinant Human DLL1 protein (ab108952)

ab108952 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108952.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"