Recombinant Human DNA Polymerase beta protein (ab111641)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP06746
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMSKRKAPQETLNGGITDMLTELANFEKNVS QAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATG KLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNED KLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSK GETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREP KDRSE
    • Molecular weight40 kDa including tags
    • Amino acids1 to 335
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab111641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab111641 is purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 30% Glycerol, 0.02% DTT, 0.58% Sodium chloride, 69% Tris HCl

General Info

  • Alternative names
    • DNA directed DNA polymerase beta
    • DNA pol beta
    • DNA polymerase beta
    • DNA polymerase beta subunit
    • DPOLB_HUMAN
    • MGC125976
    • Pol B
    • Pol beta
    • POLB
    • Polymerase (DNA directed) beta
    see all
  • FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Sequence similaritiesBelongs to the DNA polymerase type-X family.
  • DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • Post-translational
    modifications
    Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human DNA Polymerase beta protein images

  • 15% SDS-PAGE analysis of ab111641 (3 µg)

References for Recombinant Human DNA Polymerase beta protein (ab111641)

ab111641 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111641.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"