Recombinant human DNase I protein (ab73430)

Overview

Description

  • NatureRecombinant
  • SourceCHO cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight37 kDa

Specifications

Our Abpromise guarantee covers the use of ab73430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity is 1000IU/1mg. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. (Also, 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, pH 7.8 in a 50ul reaction volume.)
  • Applications

    Western blot

    Functional Studies

  • FormLiquid
  • Additional notes

     Sterile liquid colorless solution

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 8.77mg/ml Sodium chloride, 1.35mM Calcium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Deoxyribonuclease 1
    • Deoxyribonuclease I
    • Deoxyribonuclease-1
    • Deoxyribonuclease1
    • DeoxyribonucleaseI
    • DNAS1_HUMAN
    • DNASE 1
    • DNase I
    • DNase I lysosomal
    • DNASE1
    • DNaseI
    • DNL 1
    • DNL1
    • Dornase alfa
    • DRNI
    • FLJ38093
    • Human urine deoxyribonuclease I
    see all
  • FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similaritiesBelongs to the DNase I family.
  • Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt

Recombinant human DNase I protein images

  • Anti-DNase I antibody (ab97393) at 1/1000 dilution + Recombinant human DNase I protein (ab73430) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Recombinant human DNase I protein (ab73430)

ab73430 has not yet been referenced specifically in any publications.

Product Wall

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