The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ReconstitutionReconstitute with 50 µl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial. After reconsitution short term store at 4 °C, for long term storage store aliquoted at - 20 °C
Cytotoxic TRAIL receptor
Death receptor 4
NF related apoptosis-inducing ligand receptor 1
TNF-related apoptosis-inducing ligand receptor 1
TRAIL receptor 1
Tumor necrosis factor receptor superfamily member 10A
FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Tissue specificityWidely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Sequence similaritiesContains 1 death domain. Contains 3 TNFR-Cys repeats.
SDS Page analysis of ab56518; 12% separating gel; reducing conditions.
Lane 1: 2 µg,
Lane 2: 1 µg,
Lane 3: 0.5 µg.
Staining with Coomasie Blue.
Western blot - DR4 + Fc Fragment protein (ab56518)
ab56518 has a calculated molecular mass of 43 kDa. As a result of glycosylation, the recombinant protein migrates approximately as a 46 kDa protein in SDS-PAGE under reducing conditions.
Immunostaining with DR4 specific antibody, detection with Goat anti-mouse / HRP secondary antibody. Gel: 12%.
Lane 1 : ab56518 at 0.37 µg,
Lane 2 : ab56518 at 0.15 µg,
Lane 3 : ab56518 at 0.075 µg,
Lane 4 : ab56518 at 0.037 µg,
Lane 5 : ab56518 at 0.015 µg.
References for Recombinant Human DR4 protein (ab56518)
has not yet been referenced specifically in any publications.
Publishing research using ab56518? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"