The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. No detergents such as urea, Triton or Tween have been used to purify the protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
SDS-PAGE analysis of ab109838: (1) MW marker, (2) ab109838 at 3µg Gel concentration: 15%
References for Recombinant Human DTYMK protein (ab109838)
This product has been referenced in:
Su JY & Sclafani RA Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res19:823-7 (1991).
Read more (PubMed: 2017365) »
Cummins RR & Balinsky D Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. Cancer Res40:1235-9 (1980).
Read more (PubMed: 6244089) »