Overview

  • Product nameRecombinant Human DTYMK protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP23919
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK
    • Molecular weight24 kDa
    • Amino acids1 to 212
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    No detergents such as urea, Triton or Tween have been used to purify the protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Additional notes


    Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.04% DTT, 0.58% Sodium chloride

General Info

  • Alternative names
    • CDC8
    • deoxythymidylate kinase
    • Deoxythymidylate kinase (thymidylate kinase)
    • dTMP kinase
    • Dtymk
    • FLJ44192
    • KTHY_HUMAN
    • MGC198617
    • OTTHUMP00000164604
    • OTTHUMP00000200145
    • OTTHUMP00000200146
    • PP3731
    • Thymidylate kinase
    • TMPK
    • TYMK
    see all
  • FunctionCatalyzes the conversion of dTMP to dTDP.
  • PathwayPyrimidine metabolism; dTTP biosynthesis.
  • Sequence similaritiesBelongs to the thymidylate kinase family.
  • Information by UniProt

Recombinant Human DTYMK protein images

  • SDS-PAGE analysis of ab109838: (1) MW marker, (2) ab109838 at 3µg
    Gel concentration: 15%

References for Recombinant Human DTYMK protein (ab109838)

This product has been referenced in:
  • Su JY & Sclafani RA Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res 19:823-7 (1991). Read more (PubMed: 2017365) »
  • Cummins RR & Balinsky D Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. Cancer Res 40:1235-9 (1980). Read more (PubMed: 6244089) »

See all 2 Publications for this product

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