Recombinant Human dUTPase protein (ab99388)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human dUTPase protein
See all dUTPase proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab99388 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMASTVGAAGWKGELPKAGGSPAPGPETPAI SPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPP MEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVG VVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGS TGKN -
Predicted molecular weight
22 kDa including tags -
Amino acids
70 to 252 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab99388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
- Deoxyuridine triphosphatase
see all -
Function
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. -
Tissue specificity
Found in a variety of tissues. -
Pathway
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. -
Sequence similarities
Belongs to the dUTPase family. -
Post-translational
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99388 has not yet been referenced specifically in any publications.