Recombinant Human E1 Ubiquitin Activating Enzyme protein (Chemical Free) (ab80726)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Amino acids
      1 to 1058

Associated products

Specifications

Our Abpromise guarantee covers the use of ab80726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Conjugation

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.5
    Constituent: 1.19% HEPES

General Info

  • Alternative names
    • A1S9
    • A1S9 protein
    • A1S9T
    • A1S9T and BN75 temperature sensitivity complementing
    • A1ST
    • AMCX1
    • CFAP124
    • CTD-2522E6.1
    • GXP 1
    • GXP1
    • MGC4781
    • POC20
    • POC20 centriolar protein homolog
    • Protein A1S9
    • SMAX2
    • Uba1
    • UBA1, ubiquitin-activating enzyme E1 homolog A
    • UBA1_HUMAN
    • UBA1A
    • UBE 1
    • UBE 1X
    • UBE1
    • UBE1X
    • Ubiquitin activating enzyme E1
    • Ubiquitin-activating enzyme E1
    • Ubiquitin-like modifier-activating enzyme 1
    see all
  • Function
    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    ISGylated.
  • Information by UniProt

References

ab80726 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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