Recombinant Human E2F1 protein (ab82207)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected insect cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab82207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    EMSA

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • Dmel\CG6376
    • Dmel_CG6376
    • drosE2F1
    • E(Sev-CycE)3A
    • E(var)3-93E
    • E2-promoter binding facto
    • E2F 1
    • E2F transcription factor 1
    • E2F-1
    • E2f-PA
    • E2f-PB
    • E2f-PC
    • E2F1
    • E2f1 E2F transcription factor 1
    • E2F1_HUMAN
    • Evar(3)164
    • KIAA4009
    • l(3)07172
    • l(3)j3B1
    • l(3)j3C2
    • l(3)rM729
    • mKIAA4009
    • OTTHUMP00000030661
    • PBR3
    • PRB binding protein E2F 1
    • PRB-binding protein E2F-1
    • RBAP 1
    • RBAP-1
    • RBAP1
    • RBBP-3
    • RBBP3
    • RBP 3
    • RBP3
    • Retinoblastoma-associated protein 1
    • Retinoblastoma-binding protein 3
    • Transcription factor E2F1
    see all
  • FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Post-translational
    modifications
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localizationNucleus.
  • Information by UniProt

References for Recombinant Human E2F1 protein (ab82207)

ab82207 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I suggest this protein will need to be reduced and denatured before loading onto the gel. Therefore, the customer will need to add sample buffer (containing SDS and mercaptoethanol) and boil for 5 minutes, as with...

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Thank you for contacting us.

We unfotunaltery haven't tested this product in WB. Customer might have try to try the product themselves./ Please note that ab82207 is a protein not an antibody.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"