Recombinant Human EB3 protein (ab101197)

Overview

  • Product nameRecombinant Human EB3 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9UPY8
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
    • Molecular weight34 kDa including tags
    • Amino acids1 to 281
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab101197 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • APC binding protein
    • EB 3
    • EB1 protein family member 3
    • EB3
    • EBF 3
    • EBF3
    • EBF3 S
    • End binding protein 3
    • End-binding protein 3
    • MAPRE 3
    • MAPRE3
    • MARE3_HUMAN
    • Member 3
    • Microtubule Associated Protein
    • Microtubule associated protein RP/EB family member 3
    • Microtubule-associated protein RP/EB family member 3
    • OTTHUMP00000200910
    • RP 3
    • RP/EB Family
    • RP3
    see all
  • FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificityPredominantly expressed in brain and muscle.
  • Sequence similaritiesBelongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt

Recombinant Human EB3 protein images

  • 15% SDS-PAGE showing ab101197 at approximately 34.1kDa (3µg).

References for Recombinant Human EB3 protein (ab101197)

ab101197 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Unfortunately we do not sell DNA constructs.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal a...

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Thanks for your enquiry. ab101197 is our only EB3 protein product. This product is a recombinant, full length protein produced in E. coli. The SDS PAGE gel does show the protein in its monomeric form as the gel format is denaturing and reduci...

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We haven’t checked whether our protein is monomeric or dimeric.

However, we will verify it using FPLC, and within 10days we should have the results.
As soon as I have the FPLC data, I will let you know.

Thank you for contacting us.

The answers to your questions are as follows:

1) Why was DTT added?
- DTT is added because it helps stabilize proteins with free cysteines.

2) Is the protein stable without it?
- We ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"