Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      1 to 725

Specifications

Our Abpromise guarantee covers the use of ab84570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity of EEF2K was determined to be 50nmol/min/mg as per activity assay protocol
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab204858 (Myosin-2 heavy chain peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • C86191
    • Calcium/calmodulin dependent eukaryotic elongation factor 2
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
    • Calmodulin dependent protein kinase III
    • cb365
    • EC 2.7.11.20
    • eEF 2 kinase
    • eEF 2K
    • eEF-2 kinase
    • eEF-2K
    • Eef2k
    • EEF2K protein
    • EF2K_HUMAN
    • Elongation factor 2 kinase
    • Eukaryotic elongation factor 2 kinase
    • fa04b08
    • HSU93850
    • kinase eEF2K
    • MGC45041
    • SMEF2K
    • wu:fa04b08
    • zgc:100973
    see all
  • Function
    Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt

Images

  • Specific activity of EEF2K was determined to be 50nmol/min/mg as per activity assay protocol.
  • Purity of EEF2K was determined to be >80% by densitometry. approx. MW 125kDa.

References

ab84570 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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