The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 µg/µl.
EC=126.96.36.199; EC=188.8.131.52.3; EC=184.108.40.206.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
3 hydroxyacyl CoA dehydrogenase
3,2 trans enoyl CoA isomerase
Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase
L 3 hydroxyacyl CoA dehydrogenase
L bifunctional protein, peroxisomal
Peroxisomal bifunctional enzyme
Peroxisomal enoyl CoA hydratase
Liver and kidney. Lower amounts seen in the brain.
Lipid metabolism; fatty acid beta-oxidation.
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.