Recombinant human EHMT2/G9A protein (ab167966)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      RGAD VTLTDNEENI CLHWASFTGS AAIAEVLLNA RCDLHAVNYH GDTPLHIAAR ESYHDCVLLF LSRGANPELR NKEGDTAWDL TPERSDVWFA LQLNRKLRLG VGNRAIRTEK IICRDVARGY ENVPIPCVNG VDGEPCPEDY KYISENCETS TMNIDRNITH LQHCTCVDDC SSSNCLCGQL SIRCWYDKDG RLLQEFNKIE PPLIFECNQA CSCWRNCKNR VVQSGIKVRL QLYRTAKMGW GVRALQTIPQ GTFICEYVGE LISDAEADVR EDDSYLFDLD NKDGEVYCID ARYYGNISRF INHLCDPNII PVRVFMLHQD LRFPRIAFFS SRDIRTGEEL GFDYGDRFWD IKSKYFTCQC GSEKCKHSAE AIALEQSRLA RLDPHPELLP ELGSLPPVNT
    • Molecular weight
      75 kDa including tags
    • Amino acids
      807 to 1210
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab167966 was determined to be 15 nmol/min/mg as per activity assay protocol.
  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .
    Assessed by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Ankyrin repeat containing protein
    • Bat 8
    • Bat8
    • C6orf30
    • DKFZp686H08213
    • EHMT 2
    • Ehmt2
    • EHMT2_HUMAN
    • Euchromatic histone lysine methyltransferase 2
    • Euchromatic histone lysine N methyltransferase 2
    • Euchromatic histone-lysine N-methyltransferase 2
    • FLJ35547
    • G 9a
    • G9 a
    • G9A
    • G9a protein
    • G9A histone methyltransferase
    • GAT8
    • H3 K9 HMTase 3
    • H3-K9-HMTase 3
    • Histone H3 K9 methyltransferase 3
    • Histone H3 K9 methyltransferase3
    • Histone H3-K9 methyltransferase 3
    • Histone lysine N methyltransferase
    • Histone lysine N methyltransferase EHMT2
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3
    • Histone lysine N methyltransferase, H3 lysine 9 specific3
    • Histone-lysine N-methyltransferase EHMT2
    • HLA B associated transcript 8
    • HLA-B-associated transcript 8
    • KMT 1C
    • KMT1 C
    • Lysine N methyltransferase 1C
    • Lysine N-methyltransferase 1C
    • NG 36
    • NG36
    • Protein G9a
    see all
  • Function
    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • Tissue specificity
    Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    The SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • Post-translational
    modifications
    Methylated at Lys-185; automethylated.
  • Cellular localization
    Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt

Images

  • SDS Page analysis of ab167966
  • The specific activity of ab167966 was determined to be 15 nmol/min/mg as per activity assay protocol.

References

ab167966 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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