Recombinant human EHMT2/G9A protein (ab80323)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Amino acids
      785 to 1210
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab80323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 146 pmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Ankyrin repeat containing protein
    • Bat 8
    • Bat8
    • C6orf30
    • DKFZp686H08213
    • EHMT 2
    • Ehmt2
    • EHMT2_HUMAN
    • Euchromatic histone lysine methyltransferase 2
    • Euchromatic histone lysine N methyltransferase 2
    • Euchromatic histone-lysine N-methyltransferase 2
    • FLJ35547
    • G 9a
    • G9 a
    • G9A
    • G9a protein
    • G9A histone methyltransferase
    • GAT8
    • H3 K9 HMTase 3
    • H3-K9-HMTase 3
    • Histone H3 K9 methyltransferase 3
    • Histone H3 K9 methyltransferase3
    • Histone H3-K9 methyltransferase 3
    • Histone lysine N methyltransferase
    • Histone lysine N methyltransferase EHMT2
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3
    • Histone lysine N methyltransferase, H3 lysine 9 specific3
    • Histone-lysine N-methyltransferase EHMT2
    • HLA B associated transcript 8
    • HLA-B-associated transcript 8
    • KMT 1C
    • KMT1 C
    • Lysine N methyltransferase 1C
    • Lysine N-methyltransferase 1C
    • NG 36
    • NG36
    • Protein G9a
    see all
  • Function
    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • Tissue specificity
    Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    The SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • Post-translational
    modifications
    Methylated at Lys-185; automethylated.
  • Cellular localization
    Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt

Images

  • Image showing specific activity of ab80323.
  • 10% SDS-PAGE showing ab80323 at approximately 74.6kDa (5µg).

References

ab80323 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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