Recombinant Human EIF3F protein (denatured) (ab171467)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL
    • Molecular weight
      40 kDa including tags
    • Amino acids
      1 to 357
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab171467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 2.4% Urea, 0.32% Tris, 10% Glycerol

General Info

  • Alternative names
    • Deubiquitinating enzyme eIF3f
    • eIF 3 epsilon
    • eIF-3-epsilon
    • eIF3 p47
    • eIF3 p47 subunit
    • eIF3-epsilon
    • eIF3-p47
    • eIF3f
    • EIF3F_HUMAN
    • EIF3S5
    • EIF3S5, formerly
    • Eukaryotic translation initiation factor 3 subunit 5
    • Eukaryotic translation initiation factor 3 subunit 5, formerly
    • Eukaryotic translation initiation factor 3 subunit F
    • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)
    • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
    • eukaryotic translation initiation factor 3, subunit F
    • translation initiation factor 3 47 kda subunit
    see all
  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
  • Sequence similarities
    Belongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain
    The MPN domain mediates deubiquitinating activity.
  • Post-translational
    modifications
    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab171467 (3µg).

References

ab171467 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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