Additional sequence informationELK-1 (amino acids 307-428) Human Recombinant is produced in E.Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab73451 is purified by proprietary chromatographic techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized in distilled water to a concentration of 0.2-1.0 mg/mL.
ELK1 member of ETS oncogene family
ELK2 member of ETS oncogene family
ETS domain containing protein Elk 1
ETS domain containing protein Elk1
ETS domain protein Elk1
ETS domain-containing protein Elk-1
ETS like gene 1
Member of ETS oncogene family
Tyrosine kinase (ELK1) oncogene
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.
Post-translational modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.