Recombinant Human EMAP II protein (ab101823)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ12904
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMLPAVAVSEPVVLRFMIFCRLLAKMANNDA VLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLK KEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVS SGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLD LRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRM VILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAF PGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTM SNSGIK
    • Molecular weight39 kDa including tags
    • Amino acids1 to 336
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 85 % SDS-PAGE.
    ab101823 was purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

General Info

  • Alternative names
    • AIMP 1
    • Aimp1
    • AIMP1/p43
    • AIMP1_HUMAN
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
    • ARS interacting multifunctional protein 1
    • EMAP 2
    • EMAP-2
    • EMAP-II
    • EMAP2
    • EMAPII
    • Endothelial monocyte activating polypeptide
    • Endothelial monocyte activating polypeptide 2
    • Endothelial monocyte-activating polypeptide 2
    • Endothelial monocyte-activating polypeptide II
    • HLD3
    • Multisynthase complex auxiliary component p43
    • Multisynthetase complex auxiliary component p43
    • OTTHUMP00000219673
    • p43
    • Scye 1
    • Scye1
    • Scye1, formerly
    • Small inducible cytokine subfamily E member 1
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
    • Small inducible cytokine subfamily E, member 1, formerly
    see all
  • FunctionNon-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • Involvement in diseaseDefects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • Sequence similaritiesContains 1 tRNA-binding domain.
  • Post-translational
    modifications
    Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • Cellular localizationNucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt

Recombinant Human EMAP II protein images

  • 15% SDS-PAGE analysis of ab101823 (3µg)

References for Recombinant Human EMAP II protein (ab101823)

ab101823 has not yet been referenced specifically in any publications.

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