Overview

  • Product name
    Recombinant Human EME1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDIS DCEASCPPAPELFSPPVPDIAETVTQTQPVRLLSSESEDEEEFIPLAQRL TCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDT PERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTK PSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLK HIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPS EDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDI TAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQT KKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTC AFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQ QLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEG VTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD
    • Amino acids
      1 to 583
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab165544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • 6820428D13
    • Crossover junction endonuclease EME1
    • EC 3.1.22.-
    • EME1
    • Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe)
    • EME1, S. pombe, homolog of, 1
    • EME1_HUMAN
    • Essential Meiotic Endonuclease 1 Homolog 1
    • essential meiotic endonuclease 1 homolog 1 (S pombe)
    • essential meiotic endonuclease 1 homolog 2
    • Essential meiotic endonuclease 1, S. pombe, homolog of, 1
    • FLJ31364
    • hMMS4
    • homolog of yeast EME1 endonuclease
    • MGC106543
    • MMS4
    • MMS4 homolog
    • MMS4L
    see all
  • Function
    Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • Sequence similarities
    Belongs to the EME1/MMS4 family.
  • Cellular localization
    Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells.
  • Information by UniProt

Images

  • ab165544 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab165544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab165544.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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