Recombinant Human Endothelin A Receptor protein (ab114239)

Overview

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP25101
    • SpeciesHuman
    • SequenceVISDNPERYSTNLSNHVDDFTTFRGTELSFLVTTHQPTNLVLPSNGSMHN YCPQQTKITSAFK
    • Molecular weight33 kDa including tags
    • Amino acids18 to 80

Specifications

Our Abpromise guarantee covers the use of ab114239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Ednra
    • EDNRA_HUMAN
    • Endothelin 1 receptor precursor
    • Endothelin 1 specific receptor
    • Endothelin A receptor
    • Endothelin ET A receptor
    • Endothelin receptor ET1 specific type
    • Endothelin receptor subtype A
    • Endothelin receptor type A
    • Endothelin-1 receptor
    • ET A
    • ET-A
    • ETA
    • ETA R
    • ETA-R
    • ETAR
    • ETRA
    • hET AR
    • hET-AR
    see all
  • FunctionReceptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • Tissue specificityIsoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt

Recombinant Human Endothelin A Receptor protein images

  • 12.5% SDS-PAGE showing ab114239 at approximately 32.56kDa stained with Coomassie Blue.

References for Recombinant Human Endothelin A Receptor protein (ab114239)

ab114239 has not yet been referenced specifically in any publications.

Product Wall

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Both recombinant fragment peptide are validated for ELISA, but the protocol used will depend largely on the antibodies being used in the assay. The data I sent previously included relevant protocol information....

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I have forwarded all available ELISA data for ab114239.



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Thank you for your reply.


Both proteins have been used in different ELISA assays:


Regarding ab114239, this was tested in a sELISA. On the other hand, ab114259 was tested in a simple ELISA assay in which the protein was c...

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Thank you for contacting Abcam regarding the proteins ab114239 and ab114259.


I have reviewed the product specifications of these proteins as well as the kit protocols the customer is using. It is important to note that both of these pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"