Recombinant Human Endothelin B Receptor protein (ab114259)



  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP24530
    • SpeciesHuman
    • Molecular weight34 kDa including tags
    • Amino acids27 to 101

Associated products


Our Abpromise guarantee covers the use of ab114259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications



    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 µg/µl
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • ABCDS
    • Ednra
    • EDNRB
    • Endothelin B receptor
    • Endothelin B receptor precursor
    • Endothelin receptor Non selective type
    • Endothelin receptor non-selective type
    • Endothelin receptor type B
    • ET B
    • ET-B
    • ET-BR
    • ETB
    • ETBR
    • ETRB
    • Hirschsprung disease 2
    • HSCR
    • HSCR2
    • OTTHUMP00000018534
    • OTTHUMP00000178736
    • WS4A
    see all
  • FunctionNon-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificityExpressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • Involvement in diseaseDefects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • Post-translational
    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • Cellular localizationCell membrane.
  • Information by UniProt

Recombinant Human Endothelin B Receptor protein images

  • ab114259 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

References for Recombinant Human Endothelin B Receptor protein (ab114259)

ab114259 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Both recombinant fragment peptide are validated for ELISA, but the protocol used will depend largely on the antibodies being used in the assay. The data I sent previously included relevant protocol information....

Read More

Thank you for your reply.

Both proteins have been used in different ELISA assays:

Regarding ab114239, this was tested in a sELISA. On the other hand, ab114259 was tested in a simple ELISA assay in which the protein was c...

Read More

Thank you for contacting Abcam regarding the proteins ab114239 and ab114259.

I have reviewed the product specifications of these proteins as well as the kit protocols the customer is using. It is important to note that both of these pro...

Read More