Overview

  • Product name
    Recombinant Human ENO1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      msilkihare ifdsrgnptv evdlftskgl fraavpsgas tgiyealelr dndktrymgk gvskavehin ktiapalvsk klnvteqeki dklmiemdgt enkskfgana ilgvslavck agavekgvpl yrhiadlagn sevilpvpaf nvinggshag nklamqefmi lpvgaanfre amrigaevyh nlknvikeky gkdatnvgde ggfapnilen keglellkta igkagytdkv vigmdvaase ffrsgkydld fkspddpsry ispdqladly ksfikdypvv siedpfdqdd wgawqkftas agiqvvgddl tvtnpkriak avnekscncl llkvnqigsv teslqackla qangwgvmvs hrsgetedtf iadlvvglct gqiktgapcr serlakynql lrieeelgsk akfagrnfrn plak
    • Amino acids
      1 to 434

Associated products

Specifications

Our Abpromise guarantee covers the use of ab89248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab89248 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM Magnesium Sulphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2 phospho D glycerate hydro lyase
    • 2-phospho-D-glycerate hydro-lyase
    • Alpha enolase
    • Alpha enolase like 1
    • Alpha-enolase
    • C myc promoter binding protein
    • C-myc promoter-binding protein
    • EC 4.2.1.11
    • eno1
    • ENO1L1
    • ENOA_HUMAN
    • Enolase 1
    • Enolase 1 (alpha)
    • Enolase 1 (alpha) like 1
    • Enolase alpha
    • MBP 1
    • MBP-1
    • MBP1
    • MBPB1
    • MPB 1
    • MPB-1
    • MPB1
    • MYC promoter binding protein 1
    • NNE
    • Non neural enolase
    • Non-neural enolase
    • Phosphopyruvate hydratase
    • Plasminogen binding protein
    • Plasminogen-binding protein
    • PPH
    • Tau crystallin
    see all
  • Function
    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt

Recombinant Human ENO1 protein images

  • 15% SDS-PAGE showing ab89248 at approximately 47.1kDa (3µg).
  • Anti-ENO1 antibody (ab85086) at 1/1000 dilution + Recombinant Human ENO1 protein (ab89248) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant Human ENO1 protein (ab89248)

This product has been referenced in:
  • Higa LM  et al. Modulation of a-Enolase Post-Translational Modifications by Dengue Virus: Increased Secretion of the Basic Isoforms in Infected Hepatic Cells. PLoS One 9:e88314 (2014). ELISA . Read more (PubMed: 25171719) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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