• NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Associated products


Our Abpromise guarantee covers the use of ab89248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • Purity> 90 % SDS-PAGE.
    ab89248 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM Magnesium Sulphate, pH 7.5

General Info

  • Alternative names
    • 2 phospho D glycerate hydro lyase
    • 2-phospho-D-glycerate hydro-lyase
    • Alpha enolase
    • Alpha enolase like 1
    • Alpha-enolase
    • C myc promoter binding protein
    • C-myc promoter-binding protein
    • EC
    • eno1
    • ENO1L1
    • Enolase 1
    • Enolase 1 (alpha)
    • Enolase 1 (alpha) like 1
    • Enolase alpha
    • MBP 1
    • MBP-1
    • MBP1
    • MBPB1
    • MPB 1
    • MPB-1
    • MPB1
    • MYC promoter binding protein 1
    • NNE
    • Non neural enolase
    • Non-neural enolase
    • Phosphopyruvate hydratase
    • Plasminogen binding protein
    • Plasminogen-binding protein
    • PPH
    • Tau crystallin
    see all
  • FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
  • Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt

Recombinant Human ENO1 protein images

  • 15% SDS-PAGE showing ab89248 at approximately 47.1kDa (3µg).
  • Anti-ENO1 antibody (ab85086) at 1/1000 dilution + Recombinant Human ENO1 protein (ab89248) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant Human ENO1 protein (ab89248)

This product has been referenced in:
  • Higa LM  et al. Modulation of a-Enolase Post-Translational Modifications by Dengue Virus: Increased Secretion of the Basic Isoforms in Infected Hepatic Cells. PLoS One 9:e88314 (2014). ELISA . Read more (PubMed: 25171719) »

See 1 Publication for this product

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