Recombinant human Eph receptor A3 protein (ab84576)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Eph receptor A3 protein
See all Eph receptor A3 proteins and peptides -
Biological activity
Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
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Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
571 to 983
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AW492086
- Cek4
- EC 2.7.10.1
see all -
Function
Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. -
Tissue specificity
Widely expressed. Highest level in placenta. -
Involvement in disease
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
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The specific activity of Eph receptor A3 (ab84576) was determined to be 19.8 nmol/min/mg as per activity assay protocol and was equivalent to 110 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab84576
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SDS PAGE analysis of ab84576
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Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
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Purity of Eph receptor A3 was determined to be >90% by densitometry, approx. MW 72kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84576 has not yet been referenced specifically in any publications.