Recombinant human Eph receptor A3 protein (ab84576)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      571 to 983

Specifications

Our Abpromise guarantee covers the use of ab84576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AW492086
    • Cek4
    • EC 2.7.10.1
    • EK4
    • End3
    • Eph receptor A3
    • EPH-like kinase 4
    • EPH-like tyrosine kinase 1
    • EPHA3
    • EPHA3_HUMAN
    • Ephrin receptor EphA3
    • Ephrin type-A receptor 3
    • ETK
    • ETK 1
    • ETK1
    • HEK
    • HEK 4
    • HEK4
    • Human embryo kinase
    • Human embryo kinase 1
    • Mek4
    • MGC109882
    • Receptor tyrosine kinase HEK
    • Testicular tissue protein Li 64
    • Tyro 4
    • Tyro4
    • TYRO4 protein tyrosine kinase
    • Tyrosine protein kinase receptor ETK 1
    • Tyrosine-protein kinase receptor ETK1
    • Tyrosine-protein kinase TYRO4
    see all
  • Function
    Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
  • Tissue specificity
    Widely expressed. Highest level in placenta.
  • Involvement in disease
    Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt

Images

  • Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
  • Purity of Eph receptor A3 was determined to be >90% by densitometry, approx. MW 72kDa.

References

ab84576 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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