Recombinant human Eph receptor B4 protein (ab42608)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Sequenceattggaaa ctgctgatct gaagtgggtg acattccctc aggtggacgg gcagtgggag gaactgagcg gcctggatga ggaacagcac agcgtgcgca cctacgaagt gtgtgacgtg cagcgtgccc cgggccaggc ccactggctt cgcacaggtt gggtcccacg gcggggcgcc gtccacgtgt acgccacgct gcgcttcacc atgctcgagt gcctgtccct gcctcgggct gggcgctcct gcaaggagac cttcaccgtc ttctactatg agagcgatgc ggacacggcc acggccctca cgccagcctg gatggagaac ccctacatca aggtggacac ggtggccgcg gagcatctca cccggaagcg ccctggggcc gaggccaccg ggaaggtgaa tgtcaagacg ctgcgtc
    • Amino acids563 to 987

Specifications

Our Abpromise guarantee covers the use of ab42608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 125 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30?C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug /ml recombinant EPHB4.
  • Applications

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Additional notesThis protein was expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Ephb4
    • EPHB4_HUMAN
    • Ephrin receptor EphB4
    • Ephrin type-B receptor 4
    • Hepatoma Transmembrane Kinase
    • HTK
    • MYK 1
    • MYK1
    • TYRO 11
    • TYRO11
    • Tyrosine-protein kinase receptor HTK
    • Tyrosine-protein kinase TYRO11
    see all
  • FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • Tissue specificityAbundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMembrane.
  • Information by UniProt

References for Recombinant human Eph receptor B4 protein (ab42608)

ab42608 has not yet been referenced specifically in any publications.

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