Recombinant Human PICH protein (ab162850)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
Description
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Product name
Recombinant Human PICH protein -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNL DQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDA QASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSL CGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGE DEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPS SVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGV EESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLS GEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKS ADPEVMLLTLSLYKQLNNN -
Amino acids
1 to 419 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab162850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
This product was previously labelled as ERCC6L.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- ATP dependent helicase ERCC6 like
- ATP-dependent helicase ERCC6-like
- DNA excision repair protein ERCC 6 like
see all -
Function
DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats. -
Post-translational
modificationsPhosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region. -
Cellular localization
Chromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab162850 has not yet been referenced specifically in any publications.