The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is < 2.0 ng/ml, corresponding to a specific activity of > 500,000 units/mg.
% SDS-PAGE. ab116471 is purified by proprietary chromatographic techniques.
Purity is greater than 97.0% as determined by RP-HPLC and SDS-PAGE.
ab116471, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ab116471 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Please see notes section.
pH: 7.40 Constituents: PBS, 0.76% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute in sterile Ultra pure water at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.
In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon.