Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab43623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • FormLiquid
  • Additional notes

    ab43623 (Human ERK1 full length protein) can be utilized as a substrate for the following active protein kinases:

     

    ab85299 (Active human MEKK2 full length protein)

    ab55700 (Active human MAP3K8 protein fragment)

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • ERK
    • ERK 1
    • ERK-1
    • ERK1
    • ERT 2
    • ERT2
    • Extracellular Signal Regulated Kinase 1
    • Extracellular signal related kinase 1
    • Extracellular signal-regulated kinase 1
    • HGNC6877
    • HS44KDAP
    • HUMKER1A
    • Insulin Stimulated MAP2 Kinase
    • Insulin-stimulated MAP2 kinase
    • MAP Kinase
    • MAP kinase 1
    • MAP kinase 3
    • MAP kinase isoform p44
    • MAPK
    • MAPK 1
    • MAPK 3
    • MAPK1
    • Mapk3
    • MGC20180
    • Microtubule Associated Protein 2 Kinase
    • Microtubule-associated protein 2 kinase
    • Mitogen Activated Protein Kinase 3
    • Mitogen-activated protein kinase 1
    • Mitogen-activated protein kinase 3
    • MK03_HUMAN
    • OTTHUMP00000174538
    • OTTHUMP00000174541
    • p44 ERK1
    • p44 MAPK
    • p44-ERK1
    • p44-MAPK
    • P44ERK1
    • P44MAPK
    • PRKM 3
    • PRKM3
    • Protein Kinase Mitogen Activated 3
    see all
  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt

Recombinant Human ERK1 protein images

  • Anti-ERK1 antibody [M233] (ab88480) at 1/1000 dilution + Recombinant Human ERK1 protein (ab43623) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Recombinant Human ERK1 protein (ab43623)

ab43623 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"