Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      132 kDa including tags
    • Amino acids
      1 to 816

Specifications

Our Abpromise guarantee covers the use of ab126913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab126913 was determined to be 11 nmol/min/mg
  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Purity
    > 75 % Densitometry.
    The purity was determined to be >75% by densitometry
  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Big MAP kinase 1
    • BMK 1
    • BMK 1 kinase
    • BMK-1
    • BMK1
    • BMK1 Kinase
    • EC 2.7.11.24
    • ERK 4
    • ERK 5
    • ERK-5
    • ERK4
    • ERK5
    • Extracellular signal regulated kinase 5
    • Extracellular signal-regulated kinase 5
    • MAP kinase 7
    • MAPK 7
    • MAPK7
    • Mitogen activated protein kinase 7
    • Mitogen-activated protein kinase 7
    • MK07_HUMAN
    • OTTHUMP00000065906
    • OTTHUMP00000065907
    • PRKM 7
    • PRKM7
    • PROTEIN KINASE, MITOGEN-ACTIVATED, 7
    see all
  • Function
    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
  • Tissue specificity
    Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
  • Information by UniProt

Images

  • SDS-PAGE using ab126913
  • The specific activity of ab126913 was determined to be 11 nmol/min/mg

References

ab126913 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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