Recombinant Human ERp57 protein (ab92937)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL

Specifications

Our Abpromise guarantee covers the use of ab92937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab92937 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • 58 kDa glucose regulated protein
    • 58 kDa glucose-regulated protein
    • 58 kDa microsomal protein
    • Disulfide isomerase ER 60
    • Disulfide isomerase ER-60
    • Endoplasmic reticulum resident protein 57
    • Endoplasmic reticulum resident protein 60
    • ER p57
    • ER protein 57
    • ER protein 60
    • ERp 57
    • ERp57
    • ERp60
    • ERp61
    • Glucose Regulated Protein 58 Kd
    • GRP 57
    • GRP 58
    • GRP57
    • HsT17083
    • p58
    • PDIA 3
    • PDIA3
    • PDIA3_HUMAN
    • Phospholipase C alpha
    • PI PLC
    • Protein disulfide isomerase A3
    • Protein disulfide isomerase family A member 3
    • Protein disulfide-isomerase A3
    see all
  • Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant Human ERp57 protein images

  • 15% SDS-PAGE showing ab92937 (3µg) at approximately 58.5kDa.

References for Recombinant Human ERp57 protein (ab92937)

ab92937 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Functional Studies
We ordered the recombinant ERp57 for use in an experiment to examine the catalytic activity of the enzyme. Using the product provided by abcam, we were able to successful observe the catalytic reduction of insulin. Thus in our experience, this product can be used for functional studies.
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Verified customer

Submitted Apr 07 2014

Vielen Dank für Ihre Anfrage.

Leider haben wir die Aktivität dieses Produktes nicht getestet.

Da es allerdings mit 10% Glycerin und DTT versetzt wurde, gehe ich eher davon aus, dass dieses Protein nicht mehr aktiv ist.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"