Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity Assay (>90U/mg)
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity
    >= 85 % SDS-PAGE.
    Multi step chromatography
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.88% Sodium chloride, 0.03% EDTA, 0.6% Tris

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Calcium binding protein intestinal related
    • Endoplasmic reticulum resident protein 70
    • Endoplasmic reticulum resident protein 72
    • EPR70
    • EPR72
    • ER protein 70
    • ER protein 72
    • ERP 70
    • ERp 72
    • ERp-72
    • ERp70
    • ERp72
    • PDIA 4
    • Pdia4
    • PDIA4_HUMAN
    • PDIR
    • Protein disulfide isomerase A4
    • Protein disulfide isomerase associated 4
    • Protein disulfide isomerase family A member 4
    • Protein disulfide isomerase related protein
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)
    • Protein disulfide-isomerase A4
    • Protein ERp 72
    • Protein ERp72
    see all
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • SDS PAGE analysis of ab113404: Lane 1: MW Marker, Lane 2: 0.25ug, Lane 3: 0.5ug, Lane 4: 1.0ug, Lane 5: 2.5ug, Lane 6: 5.0ug
  • All lanes : an anti Human ERp72 antibody

    Lane 1 : Molecular weight ladder
    Lane 2 : Recombinant human ERp72 protein (ab113404) at 0.025 µg
    Lane 3 : Recombinant human ERp72 protein (ab113404) at 0.05 µg
    Lane 4 : Recombinant human ERp72 protein (ab113404) at 0.1 µg
  • Activity Assay: Varying amounts of ab113404 were incubated with insulin in the presence of DTT. Activity is measured as an increase in absorbance over time.

References

ab113404 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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