Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKHHHHHHAS YMSFSMKTIR LPRWLAASPT KEIQVKKYKC GLIKPCPANY FAFKICSGAA NVVGPTMCFE DRMIMSPVKN NVGRGLNIAL VNGTTGAVLG QKAFDMYSGD VMHLVKFLKE IPGGALVLVA SYDDPGTKMN DESRKLFSDL GSSYAKQLGF RDSWVFIGAK DLRGKSPFEQ FLKNSPDTNK YEGWPELLEM EGCMPPKPF
    • Molecular weight
      23 kDa including tags
    • Amino acids
      26 to 224
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Mass Spectrometry

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    > 90 % Densitometry.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 4.00
    Constituent: 0.08% Acetic acid

  • Reconstitution
    Add 200 µl of 0.1 M acetate buffer pH=4.0 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, intensive dilution by relevant buffer to a concentration of 10µg/mL recommended. In higher concentrations the solubility of this antigen is limited. Product is not sterile. Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • Cytokine-like protein EF-7
    • EF7
    • OIT1
    • Protein FAM3D precursor
    • UNQ567/PRO1130
    see all
  • Relevance
    Biological process: negative regulation of insulin secretion
  • Cellular localization
    Secreted

Images

  • 14% SDS-PAGE separation of ab168865
    Lane 1: MW marker
    Lane 2: reduced and boiled sample, 2.5 µg/lane
    Lane 3: non-reduced and non-boiled sample, 2.5 µg/lane

References

ab168865 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168865.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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