The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityInduces apoptosis of Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1µg/ml TNF Ligand enhancer. In the absence of TNF Ligand enhancer ab109359 is working at 50-100 fold higher concentrations.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab109359 can bind to human and mouse Fas.
Induces apoptosis of Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1µg/ml TNF Ligand enhancer. In the absence of TNF Ligand enhancer ab109359 is working at 50-100 fold higher concentrations.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
ReconstitutionReconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
Apoptosis (APO 1) antigen ligand 1
Apoptosis antigen ligand
Apoptosis antigen ligand 1
Fas antigen ligand
Fas ligand (TNF superfamily member 6)
Fasl Fas ligand (TNF superfamily member 6)
Generalized lymphoproliferative disease
Tumor necrosis factor (ligand) superfamily member 6
Tumor necrosis factor ligand superfamily member 6
FunctionCytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
Involvement in diseaseDefects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsN-glycosylated. The soluble form derives from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.