The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Bioactivity: Measured by ab155701 ability to inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells.
The ED50 for this effect is typically 3-20 pg/ml in the presence of 2 ng/ml recombinant Human Fas Ligand.
< 1.000 Eu/µg
>90% by SDS-PAGE. Lyophilized from 0.22 µm filtered solution
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Constituent: 99% PBS Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in 50 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Apo 1 antigen
APO 1 cell surface antigen
APO1 cell surface antigen
Apoptosis antigen 1
Apoptosis mediating surface antigen FAS
Apoptosis-mediating surface antigen FAS
CD 95 antigen
Delta Fas/APO 1/CD95
Fas (TNF receptor superfamily, member 6)
Fas cell surface death receptor
Surface antigen APO1
TNF receptor superfamily, member 6
Tumor necrosis factor receptor superfamily member 6
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.