The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Greater than 95% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 5mM
Tris, pH 8.0 to a concentration of 0.1-1.0 mg/ml. Do not
vortex. This solution can be stored at 2-8°C; for up to 1
week. For extended storage, it is recommended to further
dilute in a buffer containing a carrier protein (example
0.1% BSA) and store in working aliquots at -20°C; to -80°C;.
Fibroblast growth factor 18
FunctionPlays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.
Sequence similaritiesBelongs to the heparin-binding growth factors family.