Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionO95750
    • SpeciesHuman
    • Molecular weight28 kDa including tags
    • Amino acids1 to 216
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab108946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab108946 activates ERK and FRS2a phosphorylation in Rat hepatoma cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 90 % SDS-PAGE.
    ab108946 is 0.2µm filtered.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • FGF 19
    • FGF-19
    • FGF15
    • FGF19
    • FGF19_HUMAN
    • Fibroblast growth factor 15
    • Fibroblast growth factor 19
    see all
  • FunctionInvolved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB.
  • Tissue specificityExpressed in fetal brain, cartilage, retina, and adult gall bladder.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human FGF19 protein images

  • ERK and FRS2a phosphorylation induced by FGF19 in Rat hepatoma cells. H4IIE Rat hepatoma cells were serum starved for 16hr and then stimulated with FGF-21-Fc or FGF-b (100ng/ml) (positive control) for 10 min, respectively. Antibodies against pFRS2a, pERK1/2 & total ERK1/2 were used for immunoblotting.
    Lane 1: control (non-treated)
    Lane 2: 30ng/ml hFGF19-His
    Lane 3: 100ng/ml hFGF19-His
    Lane 4: 300ng/ml hFGF19-His
    Lane 5: 1000ng/ml hFGF19-His
    Lane 6: 100ng/ml FGF-b

References for Recombinant human FGF19 protein (ab108946)

ab108946 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"