The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab108946 activates ERK and FRS2a phosphorylation in Rat hepatoma cells.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab108946 is 0.2µm filtered.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Fibroblast growth factor 15
Fibroblast growth factor 19
FunctionInvolved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB.
Tissue specificityExpressed in fetal brain, cartilage, retina, and adult gall bladder.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
ERK and FRS2a phosphorylation induced by FGF19 in Rat hepatoma cells.
H4IIE Rat hepatoma cells were serum starved for 16hr and then stimulated with FGF-21-Fc or FGF-b (100ng/ml) (positive control) for 10 min, respectively. Antibodies against pFRS2a, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: control (non-treated)
Lane 2: 30ng/ml hFGF19-His
Lane 3: 100ng/ml hFGF19-His
Lane 4: 300ng/ml hFGF19-His
Lane 5: 1000ng/ml hFGF19-His
Lane 6: 100ng/ml FGF-b
References for Recombinant human FGF19 protein (ab108946)
has not yet been referenced specifically in any publications.
Publishing research using ab108946? Please let us know so that we can cite the reference in this datasheet.
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