Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

Overview

  • Product name
    Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active)
  • Protein length
    Full length protein
  • Description
    Recombinant human FGF21 + IgG1 protein (Fc Chimera Active)

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      50 kDa including tags
    • Additional sequence information
      Human FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.

Specifications

Our Abpromise guarantee covers the use of ab108556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    >= 95 % SDS-PAGE.
    ab108556 is 0.2 µm filtered.
  • Form
    Liquid
  • Additional notes
    Working aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localization
    FGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein

Images

  • ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
    bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: 1 µg/ml hFGF21-His (competitor)
    Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
    Lane 3: 2.2 µg/ml mCD137-Fc
    Lane 4: 100 ng/µl FGF-b
    Lane 5: control (non-treated)

References

ab108556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab108556.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up