Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

Overview

  • Product nameRecombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active)
  • Protein lengthFull length protein
  • Description
    Recombinant human FGF21 + IgG1 protein (Fc Chimera Active)

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionQ9NSA1
    • SpeciesHuman
    • Molecular weight50 kDa including tags
    • Additional sequence informationHuman FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.

Specifications

Our Abpromise guarantee covers the use of ab108556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity>= 95 % SDS-PAGE.
    ab108556 is 0.2 µm filtered.
  • FormLiquid
  • Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localizationFGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein

Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) images

  • ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
    bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: 1 µg/ml hFGF21-His (competitor)
    Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
    Lane 3: 2.2 µg/ml mCD137-Fc
    Lane 4: 100 ng/µl FGF-b
    Lane 5: control (non-treated)

References for Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

ab108556 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"