The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab108556 is 0.2 µm filtered.
Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cellular localizationFGF21: Secreted.
IgG1: Secreted. Cell membrane; Single-pass membrane protein
Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) images
Functional Studies - FGF21 protein (ab108556)
ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF
21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: 1 µg/ml hFGF21-His (competitor)
Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
Lane 3: 2.2 µg/ml mCD137-Fc
Lane 4: 100 ng/µl FGF-b
Lane 5: control (non-treated)
References for Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)
has not yet been referenced specifically in any publications.
Publishing research using ab108556? Please let us know so that we can cite the reference in this datasheet.
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