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Our Abpromise guarantee covers the use of ab54141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
Reconstituted FGF-21 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. We recommend to reconstitute it in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, further dilute the reconstituted protein in a buffer (example PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab54141 has not yet been referenced specifically in any publications.