The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab123835 has full biological activity when compared standards. The ED50, as determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts, is typically 0.25-1.25 ng/ml.
% SDS-PAGE. Purity is greater than 97% as determined by SDS-PAGE. Endotoxin Level: <0.01 ng/µg.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWhen reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. Upon reconstitution the material should be aliquoted and frozen at -20 °C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Fibroblast growth factor 4
Heparin secretory-transforming protein 1
Heparin-binding growth factor 4
Human stomach cancer transforming factor from FGF related oncogene
Kaposi Sarcoma Oncogene
Transforming protein KS3
FunctionCan transform NIH 3T3 cells from a human stomach tumor (hst) and from karposi's sarcoma (KS3). It has a mitogenic activity.
Sequence similaritiesBelongs to the heparin-binding growth factors family.