Recombinant Human Fibrillarin protein (ab99443)

Overview

  • Product name
    Recombinant Human Fibrillarin protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHRSMGKNVMVEPHRHEGVFICRGKEDALVTK NLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKP GAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTN IIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGH FVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVV VGVYRPPPKVKN
    • Molecular weight
      29 kDa including tags
    • Amino acids
      83 to 321
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    >= 85 % SDS-PAGE.
    ab99443 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • 34 kD nucleolar scleroderma antigen
    • 34 kDa nucleolar scleroderma antigen
    • Fbl
    • FBRL_HUMAN
    • FIB
    • FIB1
    • FLRN
    • Histone-glutamine methyltransferase
    • Nop1p
    • RNA U3 small nucleolar interacting protein 1
    • RNU3IP1
    • rRNA 2' O methyltransferase fibrillarin
    • rRNA 2'-O-methyltransferase fibrillarin
    see all
  • Function
    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
  • Sequence similarities
    Belongs to the methyltransferase superfamily. Fibrillarin family.
  • Post-translational
    modifications
    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
  • Cellular localization
    Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab99443 at approximately 28.9kDa (3µg).

References

ab99443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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