Recombinant human Fibroblast activation protein, alpha (ab79623)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      29 to 760
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab79623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 10.2 pmol/min/µg. Assay Condition: 50 mM Tris-HCl (pH7.5), 10 mM MgCl2, 1 mM MnCl2, 5µM substrate (A-P-AMC), room temperature.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.36% Imidazole
    Constituents: 0.64% Tris HCl, 20% Glycerol, 0.64% Sodium chloride, 0.02% Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 170 kDa melanoma membrane bound gelatinase
    • 170 kDa melanoma membrane-bound gelatinase
    • DPPIV
    • FAP
    • FAPA
    • Fibroblast activation protein alpha
    • Integral membrane serine protease
    • SEPR_HUMAN
    • Seprase
    see all
  • Function
    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificity
    Fibroblast specific.
  • Sequence similarities
    Belongs to the peptidase S9B family.
  • Post-translational
    modifications
    N-glycosylated.
    The N-terminus may be blocked.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

Recombinant human Fibroblast activation protein, alpha images

  • Image showing specific activity of ab79623.
  • SDS-PAGE showing 3ug ab79623 at approximately 85.5kDa.

References for Recombinant human Fibroblast activation protein, alpha (ab79623)

ab79623 has not yet been referenced specifically in any publications.

Product Wall



The Active human Fibroblast activation protein, alpha protein fragment (ab79623) contains a His tag at the C terminal end.

Thank you for your enquiry and interest in our products.

I would suggest loading between 1-5 ug per lane but this may need to be optimized since it depends on the detection system you will apply. Please take a look at the SDS-PAGE image on th...

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