Recombinant Human Fibronectin Fragment III.1 protein (Domain I) (ab58865)

Overview

  • Product name
    Recombinant Human Fibronectin Fragment III.1 protein (Domain I)
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Additional sequence information
      Fibronectin III.1 domain.

Specifications

Our Abpromise guarantee covers the use of ab58865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None

  • Reconstitution
    Reconstitute with 0.5 ml of sterile PBS per 0.5 mg vial. The stock solution can be further diluted with sterile PBS or cell culture medium.

General Info

  • Alternative names
    • CIG
    • Cold insoluble globulin
    • Fibronectin 1
    • Fibronectin precursor
    • FINC
    • FN
    • LETS
    • Migration stimulating factor
    • MSF
    see all
  • Relevance
    Fibronectins are made of two subunits linked by disulfide bonds at the C terminal. In the extracellular matrix fibrils, fibronectins are further disulfide bonded into high molecular weight polymers. Fibronectin subunits vary in size between approximately 235 and 270 kDa depending on tissue and species. Each subunit is made of repeating modules of three types: I, II, and III. There are 12 type I repeats, approximately 45 amino acids long, clustered in three groups, two adjacent type II repeats each 60 amino acids long, and 15-17 type III repeats each about 90 amino acids long. Type I and type II each contains two disulfide bonds, while type III lacks disulfide bonds. There are two free sulfhydryl groups per subunit at the type III repeat. Recombinant fragment III1-C, modeled after the C-terminal two-thirds of the III1 repeat, was found to bind to fibronectin and induce spontaneous disulfide crosslinking of the fibronectin molecules into multimers, which resemble matrix fibrils.(Moria, A., et al., Nature, 367, 193 (1994).
  • Cellular localization
    Secreted protein; extracellular space; extracellular matrix

References

ab58865 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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