Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9NWM8
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMALIPEPEVKIEVLQKPFICHRKTKGGDLM LVHYEGYLEKDGSLFHSTHKHNNGQPIWFTLGILEALKGWDQGLKGMCVG EKRKLIIPPALGYGKEGKGKIPPESTLIFNIDLLEIRNGPRSHESFQEMD LNDDWKLSKDEVKAYLKKEFEKHGAVVNESHHDALVEDIFDKEDEDKDGF ISAREFTYKHDEL
    • Molecular weight24 kDa including tags
    • Amino acids20 to 211
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP14 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab99328 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 22 kDa FK506 binding protein
    • 22 kDa FK506-binding protein
    • 22 kDa FKBP
    • FK506 binding protein 14
    • FK506 binding protein 14 (22 kDa)
    • FK506-binding protein 14
    • FKB14_HUMAN
    • FKBP 22
    • FKBP-14
    • FKBP-22
    • FKBP14
    • FKBP22
    • FLJ20731
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl-prolyl cis-trans isomerase FKBP14
    • PPIase
    • PPIase FKBP14
    • Rotamase
    see all
  • FunctionPPIases accelerate the folding of proteins during protein synthesis.
  • Involvement in diseaseEhlers-Danlos syndrome, with progressive kyphoscoliosis, myopathy, and hearing loss
  • Sequence similaritiesContains 2 EF-hand domains.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt

Recombinant human FKBP14 protein images

  • 15% SDS-PAGE analysis of 3µg ab99328.

References for Recombinant human FKBP14 protein (ab99328)

ab99328 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99328.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"