Recombinant human FKBP1B protein (ab113596)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHRSMGVEIETISPGDGRTFPKKGQTCVVHYT GMLQNGKKFDSSRDRNKPFKFRIGKQEVIKGFEEGAAQMSLGQRAKLTCT PDVAYGATGHPGVIPPNATLIFDVELLNLE
    • Molecular weight
      14 kDa including tags
    • Amino acids
      1 to 108
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP1B protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    SDS-PAGE

    Functional Studies

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab113596 was purified using conventional chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 12.6 kDa FK506-binding protein
    • 12.6 kDa FKBP
    • Calstabin 2
    • FK506 binding protein 1 like
    • FK506 binding protein 12.6
    • FK506 binding protein 1B
    • FK506 binding protein 1B 12.6 kDa
    • FK506-binding protein 1B
    • FKB1B_HUMAN
    • FKBP 12.6
    • FKBP 1B
    • FKBP 1L
    • FKBP 9
    • FKBP-12.6
    • FKBP-1B
    • FKBP12.6
    • Fkbp1b
    • FKBP1L
    • FKBP9
    • h FKBP 12
    • h-FKBP-12
    • Immunophilin FKBP12.6
    • OTK 4
    • OTK4
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl prolyl cis trans isomerase 1B
    • Peptidyl-prolyl cis-trans isomerase FKBP1B
    • PKBP 1L
    • PKBP1L
    • PPIase
    • PPIase 1B
    • PPIase FKBP1B
    • Rotamase
    • Rotamase 1B
    see all
  • Function
    Associates with the ryanodine receptor (RYR-2) in cardiac muscle sarcoplasmic reticulum and may play a unique physiological role in excitation-contraction coupling in cardiac muscle. There are four molecules of FKBP12.6 per heart muscle RYR. Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Tissue specificity
    Isoform 1 and isoform 2 are Ubiquitous with highest levels in brain and thymus.
  • Sequence similarities
    Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab113596 (3µg)

References

ab113596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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