The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological activity: Specific activity is > 490 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
Add 10 µl of recombinant FKBP3 protein with 1 µg in assay buffer.
Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
Record the increase in A405 nm for 30 minutes at 25°C.
% SDS-PAGE. ab93683 is purified using conventional chromatography techniques.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
25 kDa FK506-binding protein
25 kDa FKBP
FK506 binding protein 25 T cell
FK506 binding protein 25, T cell, 25-KD
FK506 binding protein 3 (25kD)
FK506-binding protein 3
Peptidyl-prolyl cis-trans isomerase FKBP3
Rapamycin binding protein
Rapamycin-selective 25 kDa immunophilin
FunctionFK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
Sequence similaritiesBelongs to the FKBP-type PPIase family. Contains 1 PPIase FKBP-type domain.