The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: 20 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30 C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant Flt3.
Additional notesThis protein was expressed in a Baculovirus infected Sf9 cell expression system. Expected molecular weight of 54.2 kDa.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Fetal liver kinase 2
FL cytokine receptor
FMS like tyrosine kinase 3
Fms related tyrosine kinase 3
Fms-like tyrosine kinase 3
Growth factor receptor tyrosine kinase type III
Receptor type tyrosine protein kinase FLT3
Stem cell tyrosine kinase 1
Tyrosine protein kinase receptor FLT3
Tyrosine-protein kinase receptor FLT3
FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
Tissue specificityBone marrow cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 protein kinase domain.