Recombinant Human FOXA2 protein (ab95848)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      48 kDa
    • Additional sequence information
      There is no epitope tag.

Specifications

Our Abpromise guarantee covers the use of ab95848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Indirect ELISA

  • Purity
    > 90 % SDS-PAGE.
    ab95848 was refolded to soluble form.
  • Form
    Liquid
  • Additional notes
    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 500mM L-arginine, 300mM Guanidine-HCl, 50mM HEPES, 5mM Cystamine, 5mM Cysteamine, 5mM Calcium chloride, 3mM DTT, pH 7.5

General Info

  • Alternative names
    • Forkhead box A2
    • Forkhead box protein A2
    • FOX A2
    • foxa2
    • FOXA2_HUMAN
    • Hepatic nuclear factor 3 beta
    • Hepatocyte nuclear factor 3
    • Hepatocyte nuclear factor 3 beta
    • Hepatocyte nuclear factor 3-beta
    • HNF 3B
    • HNF-3-beta
    • HNF-3B
    • HNF3B
    • MGC19807
    • TCF 3B
    • TCF-3B
    • TCF3B
    • Transcription factor 3B
    see all
  • Function
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt

Images

  • SDS-PAGE analysis of 1.5µg ab95848.
  • anti-FOXA2 monoclonal at 1 µg/ml + Recombinant Human FOXA2 protein (ab95848) at 0.075 µg
  • Indirect ELISA detection of immobilized ab95848 with titrated amounts of FOXA2 monoclonal antibody.

References

ab95848 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
SDS-PAGE
Cooking the Human FOXA2 full length protein, and run the SDS gel. Use the anti-foxa2 (R&D) to detect it
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Verified customer

Submitted Aug 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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