Recombinant Human FPR1 co-expressed with Gia2, ß1 and gamma 2 protein (ab90417)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab90417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes


    Human FPR1 exists in various isoforms, FPR-26, FPR-98 and FPR-G6, respectively. These FPR isoforms differ from each other in amino acid positions 101 (localized at the top of the third transmembrane domain), 192 (localized in the center of the second extracellular loop) and 346 (localized at the extreme C-terminus). (FPR-26: V101, N192, E346; FPR-98: L101, N192, A346; FPR-G6: V101, K192, A346). ab90417 (FPR-G6) exhibits a partial Gi-protein coupling defect relative to FPR-26.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

General Info

  • Alternative names
    • Adenylate cyclase-inhibiting G alpha protein
    • fMet-Leu-Phe receptor
    • FMLP
    • fMLP receptor
    • Formyl peptide receptor 1
    • FPR
    • GNAI2B
    • Guanine nucleotide-binding protein G(i) subunit alpha-2
    • Lipoxin A4 receptor
    • LXA4R
    • N-formyl peptide receptor
    • N-formylpeptide chemoattractant receptor
    • OTTMUSP00000024381
    see all
  • RelevanceFMLP (Formyl peptide receptor 1), a Chemoattractant Receptor, mediates chemotaxis, degranulation, and superoxide production, as part of the inflammatory response. Bacterial N-formylmethionyl peptides and Annexin A1, specific ligands for FMLP, attract polymorphonuclear neutrophils to sites of infection. FMLP receptors promote the phosphorylation and downregulation of CCR5, which has been shown to inhibit HIV infection. Therefore, ligands for an FMLP receptor may be able to inhibit HIV infection. FMLP has been reported in blood monocytes, neutrophils, and phagocytes, as well as in liver, astrocytes, and microglia. ESTs have been isolated from B-cell/lung/testis, blood, brain, eye, heart, liver/spleen, kidney, lung, pancreas, placenta, spleen, and uterus libraries. The G protein family of signal transducers includes 5 heterotrimers, which are most clearly distinguished by their different alpha chains; they have virtually identical beta chains and similar gamma chains. G protein alpha inhibitor chain 2 is a subunit of the G(i) G protein, whose role is in inhibiting adenylate cyclase activity in response to bound GTP. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
  • Cellular localizationCell membrane; Multi-pass membrane protein.

References for Recombinant Human FPR1 co-expressed with Gia2, ß1 and gamma 2 protein (ab90417)

ab90417 has not yet been referenced specifically in any publications.

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