Recombinant Human FTO protein (ab109039)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9C0B1
    • SpeciesHuman
    • Molecular weight65 kDa including tags
    • Amino acids2 to 505
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level< 1.000 Eu/µg
  • Purity> 90 % SDS-PAGE.
    ab109039 is 0.2µm filtered.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 55mM Tris HCl, 150mM Sodium chloride, pH 8.2

General Info

  • Alternative names
    • AlkB homolog 9
    • ALKBH9
    • Alpha-ketoglutarate-dependent dioxygenase FTO
    • AW743446
    • Fat mass and obesity-associated protein
    • FATSO, MOUSE, HOMOLOG OF
    • Fto
    • FTO_HUMAN
    • GDFD
    • KIAA1752
    • mKIAA1752
    • Protein fatso
    see all
  • FunctionDioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • Tissue specificityUbiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • Involvement in diseaseDefects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • Sequence similaritiesBelongs to the fto family.
  • DomainThe 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • Cellular localizationNucleus.
  • Information by UniProt

References for Recombinant Human FTO protein (ab109039)

ab109039 has not yet been referenced specifically in any publications.

Product Wall

At this time we have not determined it's enzymatic activity. Also, cleavage of the His-tag is not possible.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"