Recombinant human Fyn protein (ab84696)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab84696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: 166nmol/min/mg.
  • Applications

    Functional Studies

    Other

    Western blot

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • C syn protooncogene
    • Fyn
    • FYN oncogene related to SRC FGR YES
    • FYN_HUMAN
    • OKT3 induced calcium influx regulator
    • P59 FYN
    • p59-Fyn
    • Protein tyrosine kinase fyn
    • Proto oncogene tyrosine protein kinase fyn
    • Proto-oncogene c-Fyn
    • Proto-oncogene Syn
    • Protooncogene Syn
    • SLK
    • Src like kinase
    • Src yes related novel gene
    • Src-like kinase
    • Src/yes related novel
    • SYN
    • Tyrosine kinase p59fyn T
    • Tyrosine kinase p59fyn(T)
    • Tyrosine-protein kinase Fyn
    see all
  • FunctionTyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificityIsoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localizationCell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

Recombinant human Fyn protein images

  • SDS Page analysis of ab84696

References for Recombinant human Fyn protein (ab84696)

ab84696 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.
The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
Therefore the band at ca 85...

Read More

Thank you for contacting Abcam.
The activity of this protein is 166nmol/min/mg and was assessed using the gold standard radiometric approach where a radio-labeled p33 was used to quantify the phosphorylation of the kinase to its substrate.
The ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"