Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      AGPATQSPMK LMALQLLLWH SALWTVQEAT PLGPASSLPQ SFLLKCLEQV RKIQGDGAAL QEKLCATYKL CHPEELVLLG HSLGIPWAPL SSCPSQALQL AGCLSQLHSG LFLYQGLLQA LEGISPELGP TLDTLQLDVA DFATTIWQQM EELGMAPALQ PTQGAMPAFA SAFQRRAGGV LVASHLQSFL EVSYRVLRH
    • Molecular weight
      21 kDa
    • Amino acids
      31 to 204

Specifications

Our Abpromise guarantee covers the use of ab167700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The bio-activity was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. ED50<0.05ng/ml, corresponding to a specific activity of >2X107 Unit/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 94% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • C17orf33
    • Colony stimulating factor 3
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3_HUMAN
    • CSF3OS
    • Csfg
    • Filgrastim
    • G-CSF
    • GCSA
    • GCSF
    • Granulocyte colony stimulating factor
    • Granulocyte colony-stimulating factor
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • Pluripoietin
    see all
  • Function
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE of reduced ab167700 stained overnight with Coomassie Blue.

References

ab167700 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab167700.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up