Overview

  • Product nameRecombinant Human G3BP protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ13283
    • SpeciesHuman
    • SequenceMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGK PADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSN NNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESE EEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEP EPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFS WASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHE VDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPI MFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM VQKPGFGVGRGLAPRQVEHHHHHH
    • Molecular weight53 kDa including tags
    • Amino acids1 to 466
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab103304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 85 % SDS-PAGE.
    ab103304 was purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8.0

General Info

  • Alternative names
    • ATP dependent DNA helicase VIII
    • ATP-dependent DNA helicase VIII
    • G3BP
    • G3BP-1
    • G3bp1
    • G3BP1_HUMAN
    • GAP binding protein
    • GAP SH3 domain binding protein 1
    • GAP SH3 domain-binding protein 1
    • GTPase activating protein (SH3 domain) binding protein 1
    • hDH VIII
    • Human DNA helicase VIII
    • MGC111040
    • Ras GTPase activating protein binding protein 1
    • Ras GTPase activating protein SH3 domain binding protein
    • Ras GTPase-activating protein-binding protein 1
    • RasGAP associated endoribonuclease G3BP
    see all
  • FunctionMay be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe NTF2 domain mediates multimerization.
  • Post-translational
    modifications
    Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt

Recombinant Human G3BP protein images

  • 15% SDS-PAGE analysis of 3µg ab103304.

References for Recombinant Human G3BP protein (ab103304)

ab103304 has not yet been referenced specifically in any publications.

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